Coding
CirCir

Part:BBa_K1392930:Design

Designed by: Burak Kızıl   Group: iGEM14_METU_Turkey   (2014-09-29)


2-hydroxy-6-oxohepta-2,4-dienoate hydrolase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 309
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 309
    Illegal NotI site found at 213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 309
    Illegal BamHI site found at 266
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 309
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 309
    Illegal NgoMIV site found at 76
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 52


Design Notes

2-hydroxymuconate semialdehyde has cytotoxic effects on a cell but with the expession of 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase, in pet28a vector, we are planning to degredate it into 2-oxopent-4-enoate and it is the first step that goes to the pyruvate metabolysm.


Source

Pseudomonas putida (F1 Strain)

References

http://www.ncbi.nlm.nih.gov/gene?LinkName=pubmed_gene&from_uid=1916282 http://www.ncbi.nlm.nih.gov/pubmed/?term=1916282